|Session times: 9:15-10:45 | 11:00-12:30 | 1:30-3:00 | 3:15-4:45|
Software dongles are available for loan, but must be signed out in the relevant logbook. The dongles can be found hanging above the hotdesk (2-42-N).
Please do not hold these dongles overnight unless confirmed with a Detmold Staff member that the dongle is not required for installation or training in the morning. Please do not take these dongles off site, i.e. they are never to be taken home for use. If the dongle is lost the cost of replacing the software licence will be billed according to its loss.
A fair use policy applies to all of the software dongles. Please be aware that Detmold Staff may contact you for return of the dongle if your usage has exceeded a fair limit.
|FCS express v6 New||This is the best package for offline analysis of flow data from any analyser or sorter, this licence includes the modules for cell cycle analysis and proliferation analysis. Contact Kate for a demo!|
|FCS express v4||Older version of FCS express (replaced by v6 in Jan 2017). Does not have access to additional modules.|
|FCAP Array||Software for analysis of Cytometric Bead Arrays (CBA) from BD|
|Kaluza||Software package released by Beckman Coulter for offline analysis|
|FlowJo (not available)||Sorry, there are no plans to acquire a licence. You will need to source your own licence if required.|
This badboy has 5 lasers (355nm, 405nm, 488nm, 561nm, 640nm). It can analyse 20 fluorochromes.
Sorting formats: 6-way 5 mL-tube, plate-sorting and small particle sorting.
|Beckman Coulter Astrios page||Link|
|Astrios fluorochrome options||Link to manufacturer|
|355 nm||448/59||595 LP (deflected)|
|620/29||595 LP (p) > 650 SP (p)|
|692/75||595 LP (p) > 650 SP (d)|
|405 nm||405/10||506 LP (d) > 418 LP (d)|
|448/50||506 LP (d) > 418 LP (p)|
|546/20||506 LP (passthrough)|
|488 nm||488/6||642 LP (d) > 595 LP (d) > 558 SP (p) > 495 SP (p)||SSC|
|513/26||642 LP (d) > 595 LP (d) > 558 SP (p) > 495 SP (d)|
|576/21||642 LP (d) > 595 LP (d) > 558 SP (d)|
|620/29||642 LP (d) > 595 LP (p)|
|664/22||642 LP (p) > 681 LP (d)|
|710/45||642 LP (p) > 681 LP (p) > 748 SP (p)|
|797/70||642 LP (p) > 681 LP (p) > 748 SP (d)|
|561 nm||561/4||595 LP (d) > 557 LP (p)||SSC|
|579/16||595 LP (d) > 557 LP (p)|
|614/20||595 LP (p) > 642 LP (d)|
|692/75||595 LP (p) > 642 LP (p)|
|640 nm||642/10||759 LP (d) > 699 LP (d) > 650 SP (p)|
|671/30||759 LP (d) > 699 LP (d) > 650 SP (d)|
|722/44||759 LP (d) > 699 LP (p)|
|797/70||759 LP (p)|
This machine is no longer onsite, so arrangements should be made to run samples on the Astrios instead.
The Aria has 3 lasers (407nm, 488nm, 633nm) and is capable of analysing 8 fluorochromes simultaneously.
Sorting formats: 4-way 5 mL-tube and plate-sorting.
The lack of a 561-laser means that experiments involving RFP/mCherry should be run on the Astrios instead.
|407 nm||-||450/40||Alexa Fluor 405, BV421, Cascade Blue, CFP, DAPI, Hoechst, Hoechst Blue, Indo-1 (Blue or Violet), Pacific Blue, V450, BFP, Cerulean, eCFP, Hoescht 33342, Live/Dead Violet|
|502||530/30||Alexa Fluor 430, BV510, Pacific Orange, V500, Krome Orange, Live/Dead Aqua, SYTOX Blue|
|690||710/50||BV650, BV711, BV786, Hoechst Red, Qdot 525, Qdot 705, Qdot 800, FG, FluoroGold, UVR|
|502||530/30||Alexa Fluor 488, FITC, GFP, Calcein, CFSE, Cy2, DyLight 488, Emerald, Live/Dead Green, SYBR Green, YFP|
|556||585/42||PE, Alexa Fluor 546, Alexa Fluor 555|
|610||616/23||PE-Texas Red, Alexa Fluor 568, BD Horizon PE-CF594, Belgian Red, ECD, Propidium Iodide|
|655||695/40||7-AAD, PE-Cy5, PE-Cy5.5, PerCP, PerCP-Cy5-5, PI, PerCP-eFluor 710, PerCP-Vio770|
|633 nm||-||660/20||Alexa Fluor 680/700/750, APC-Cy7, APC-H7, APC-AlexaFluor 700/750/780, APC-Vio770|
|735||780/60||APC, Alexa Fluor 647, Alexa 647, Alexa Fluor 633, Cy5, Cy5-5, DRAQ5, Live/Dead Far Red|
Manufacturer links have been depreciated. However, there are resources available online via your favourite search engine (search for: FACSAria II).
The LSM 700 confocal microscope is located in the Detmold facility. For access and training please contact us.
The LSM 710 two-photon microscope is located in the basement. Please contact Claudine Bonder for access and training.
The Leica SP8 is located on the third floor. Please contact Michael Samuel for access and training.
The histech confocal slide scanner is located in the genomics facility (0-46-N). Please contact Michael Samuel for access and training.
The LSM 800 confocal microscope is located in the Detmold facility. For access and training please contact us.